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Related post: of endogenous retroviruses in mice and the mechanisms of their regulation,
expression, and in the case of the recombinant (MCF) viruses, their generation.
These projects, utilizing biological, genetic, serological, and molecular
approaches, have helped to unravel some of the details of lymphomagenesis in
AKR mice and have defined many of the biological properties of MCF viruses.
The studies this year have focused on 1) Further biological characterization
of MCF viruses in the determination of their host range, 2) Detailed genetic
analysis of host regulation Order Coumadin Online of AKR MCF induction of lymphoma, 3) Cell-virus
interactions in vivo using lymphomagenic and nonlymphomagenic AKR-type eco-
tropic viruses and intragenomic recombinants constructed between them, 4)
Examination of the genetic sequences of the mouse which recombine with dif-
ferent ecotropic Coumadin Online viruses to generate MCF viruses, and 5) Differential viral
gene expression of leukemia Buy Coumadin cells as reflected by env antigen presentation.
Major findings from this work include: 1) The host range of MCF viruses was
found to be distinct from that of other classes of murine retroviruses, which
supported the recent Order Coumadin contention that MCF's are a distinct virus class, 2) Gene
conveying resistance to MCF induction of lymphoma was conclusively demonstrated
in crosses of AKR with NFS, 3) The promotor region of the genome of lympho-
magenic AKV-type virus was shown to be the important element for cellular
specificity, replication, and oncogenicity, 4) Ecotropic viruses recombine in
a specific manner with different proviral sequences of the mouse to generate
MCF viruses, and 5) MCF viral env gene products and not xenotropic were ex-
pressed on spontaneous or virus-induced leukemias and the type Buy Coumadin Online of MCF protein
was determined by the mouse strain or ecotropic virus used. ^
PHS 6040 (Rev. 1/83)
Rocky Mountain Laboratories
1983 Annual Report
Table of Contents
Natural History of Tick-Borne Rickettsia
and Their Public Health Significance - Burgdorfer
Immune Responses to Rickettsial Infections -
Peacock and Thomas
Antigens and Classification of the Rickettsiae -
Philip Generic Coumadin and Anacker (terminated) (See LMSF Annual
Report) 29- 9
Systematics and Vector Relationships of Certain
Parasitic Arthropods - Clifford 29-10
The Epidemiology of Human Infections of Special
Interest - Philip (terminated) 29-11
Relation of Viruses to the Genesis of Chronic
Disease - Had low 29-12
Vector-Pathogen Relationships - Yunker (terminated) 29-13
Immunobiology of Relapsing Fever - Stoenner
Ecology of Buy Cheap Coumadin Lyme Disease and Related Disorders Cheap Coumadin -
Rocky Mountain Laboratories
National Institute of Allergy and Infectious Diseases
October 1, 1982, to September 30, 1983
The June 18, 1982, Science Report on our discovery of the Lyme disease agent
and on the application of the indirect immunofluorescence test as a diagnostic
tool Purchase Coumadin for Lyme disease and related disorders became the subjects of numerous
press articles that hailed our findings as a significant and exciting break-
through in medical research. This was echoed in this country as well as in
Europe by research scientists and clinicians (dermatologists, rheumatologists)
who were eager to either obtain subcultures of the causative agent for their
own research and diagnostic purposes or to offer for serologic testing sera of
patients with suspected Lyme disease.
As anticipated, our findings also rekindled interest Purchase Coumadin Online for epidemiological and
The following sections summarize this year's developments in our research
program. Some of the findings were reported in 18 publications and in an
additional 16 papers that are currently in press.
As in previous years, we functioned as a World Health Organization Center for
Rickettsial Reference and Research and provided reference reagents, guidance
and training to investigators and staff of various domestic and foreign health
Comparative immunochemical analyses (SDS-PAGE, western blot) between the "Swiss
agent", a spotted fever group rickettsia isolated from up to 12% of Ixodes
ricinus from Switzerland, and the three in Europe prevalent spotted fever group
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